Guide Label-Free Biosensors: Techniques and Applications

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The lack of rapid, sensitive, multiplexed time-course cytokine monitoring techniques poses significant challenges to these research efforts. To fill this technological gap, this thesis has developed label-free biosensing platforms for rapid and sensitive cytokine detection. In the first part of this thesis, an MoS2-based field-effect transistor FET biosensor was developed to detect cytokines.

Label-free optical biosensor OWLS 210

In the second part, we demonstrated a nanoparticle based localized surface plasmon resonance LSPR biosensing device integrated with microfluidic technology. The quantitative characterization of cytokine secretion behaviors from T-cells under an immunomodulation was studied at high temporal resolution.

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The biosensors achieved precise measurements with low sample volume, short assay time, high-sensitivity, and negligible sensor crosstalk. Data obtained from this study provided a comprehensive picture of the time-varying cellular functional response during immunosuppression.

The combination of label-free optical biosensors and FluidFM BOT for cellular studies

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Label-free optical biosensors

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